Chromosome walking and gene cloning using a Neurospora crassa Linkage Group VI-specific library.
نویسندگان
چکیده
منابع مشابه
A Partial Map of Linkage Group D in Neurospora Crassa.
chromosomes are rather small, so it is quite likely that minor chromosome deficiencies or deletions would not be detected. Recently, we have obtained from commercial sources two spontaneous chromosome aberrants, one of which appears to be wholly tetraploid, while the other is chimaeric, containing diploid as well as tetraploid tissues. The first named is known to be, and the other probably is, ...
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We have constructed a genomic library of Neurospora crassa DNA in a cosmid vector that contains the dominant selectable marker for benomyl resistance. The library is arranged to permit the rapid cloning of Neurospora genes by either sib-selection or colony-hybridization protocols. Detailed procedures for the uses of the library are described. By use of these procedures, a modest number of unrel...
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N 1946 a tentative chromosome map of Neurospora crassa was published I (BEADLE) showing members, among the biochemical mutants obtained a t the Stanford laboratory, of the five linkage groups then known. The data have not been published since they were considered not sufficiently extensive, in most cases, to establish relative positions of the various genes within the linkage groups. Since that...
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A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects are not limited to genes involved in phosphorus metabolism. In addition, we have characterized a nu...
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HE purpose of this paper is to provide evidence for the establishment of one Tor more genetic markers on seven linkage groups in Neurospora tetrasperma so as to enhance the usefulness of this species in genetic research. The linkage groups were determined by hybridization with the genetically well known species, N . crmsa. Markers were first transferred from one Neurospora species to another by...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1999
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1237